Alkaliphiles: Genetic Properties and Applications of Enzymes by Koki Horikoshi PDF

By Koki Horikoshi

ISBN-10: 354033372X

ISBN-13: 9783540333722

The pioneering paintings of Dr. Koki Horikoshi at the discovery, examine and functions of alkaliphilic micro organism are defined the following of their entirety. The isolation, distribution and taxonomy of alkaliphilic microorganisms in addition to their cellphone constitution and body structure are mentioned for a easy realizing of those entities. The molecular biology and genome sequencing of a few alkaliphilic bacterial lines also are presented.

Part II of the quantity makes a speciality of enzymes of alkaliphiles and their purposes. those comprise alkaline proteases, starch-degrading enzymes and various others. a few of these enzymes are at the moment in large advertisement use as laundry detergent ingredients and in wastewater treatment.

Alkaliphiles are a comparatively contemporary box of study that might without doubt supply avenues to a variety of additional discoveries and purposes for a brand new new release of employees in bioscience and technology.

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Extra resources for Alkaliphiles: Genetic Properties and Applications of Enzymes

Example text

BactmoL, 185, 5133(2003)) the step stimulated by Na ^ and K ^, these ions stimulate the uptake of the germinants into the spores. 3 Temperature and Nutrition High temperature for growth is not a characteristic property of alkaliphiles. PO4 Glucose MgCl, CaCf. 8 clausii No. 221 (ATCC 21522), which is an alkahne protease producer. Kimura and Horikoshi (1988) isolated several psychrophilic alkaliphiles. 5. 0 at 20°C. But almost all alkaliphilic bacteria showed optimum growth at 25-45°C. No precise experiment has been reported on nutrient requirements.

And it has a slightly higher pH optimum than the E. eoli enzyme. Further role for alkaliphily was not discussed. In 1999, the effect of growth pH on the phospholipid contents of the membranes from alkaliphilic bacteria was investigated by Enomoto and Koyama (1999). An aerobic alkaliphile Baeillus sp. 5. 5. 5, Phospholipids of the membranes from YN-2000 and BL77/1 were composed mainly of cardiolipin (CL), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). It is suggested that the increases by growth at pH 10 of negative charges on the membranes from the bacteria result mainly from the increases of acidic phospholipids such as CL and PG.

Flagella are typically as long as or longer than the cell body, varying in length from one to many micrometers. Several alkaliphilic bacteria demonstrate vigorous motility at alkaline pH. Flagella exposed to alkaline environments and devices for movement may operate at alkaline pH. 1 Flagella Formation and Flagellin Aono et al. (1992a) investigated pH-dependent flagella formation by facultative alkaliphilic Bacillus halodurans C-125. This strain grown at alkaline pH had peritrichous flagella and was highly motile.

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Alkaliphiles: Genetic Properties and Applications of Enzymes by Koki Horikoshi


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